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The purified GST- jr PPO1-wt fusion protein was exchanged to a glutathione-free buffer containing 200 mM NaCl and 50 mM Tris-HCl at pH 7.8 and 1 mM dithiothreitol (DTT) using a Vivaspin ultrafiltration device (VWR, molecular weight cut-off 30 kDa)

Additionally, dopamine 1 receptor (SMD = 5.05, 95% CI 6.14 to 3.97) and dopamine 2 receptor (SMD = 1.13, 95% CI 1), levels were found to be lower in patients with AD, when compared to normal individuals are indicated

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